Responsible: Maria Isabel Sierra Alonso Address: C/ Tulipán s/n. Mostoles 28933 Department: Chemical and Environmental Technology Phone: (914) 887 018 |
Essays/Services offered
- Optimization of preparation protocols for environmental samples, drugs, food, chemical industry products, etc. for analysis (sieving, filtration, LLE, SLE, digestion, SPE, dSPE, microextractive techniques such as μSPEed, MSPE, etc.).
- Optimization and validation of chromatographic, spectrophotometric and electrochemical analytical methods.
- Separation and quantification of chiral and non-chiral compounds (alkaloids and other natural toxins, phenolic compounds and other bioactives, antibiotics, beta-blockers and other compounds of pharmacological interest, phytosanitary compounds, priority and emerging contaminants, etc.) by HPLC in environmental samples, foods, drugs, chemical industry products, etc., with UV-Vis detection, fluorescence and tandem mass spectrometry.
- Analysis of samples by UV-Vis spectrophotometry. Measurements of total phenolic compounds (Folin-Ciocalteu method), total anthocyanins, flavonoids, antioxidant activity (DPPH, ABTS, FRAP), etc.
- Determination of organic and inorganic contaminants (bisphenol A, alkaloids, heavy metals, etc.) in aqueous samples using electrochemical techniques such as voltammetry in all its variants (DPV, CV, SWV), amperometry, etc. using modified carbon electrodes, screen printed graphite, gold, etc.
- Determination of textural properties in solids (BET area, Langmuir surface area, pore volume, adsorption and desorption isotherms).
- Optimization and validation of catalytic and photocatalytic reaction protocols in organic or aqueous media using UV or visible irradiation systems
- Separation and quantification of compounds from catalytic and photocatalytic reactions in organic and aqueous media using HPLC with UV-Vis detection.
- Separation and quantification of compounds from catalytic and photocatalytic reactions using GC with FID or mass spectrometry detection.
- Analysis of organic and inorganic catalysts in liquid or solid state using FTIR, UV-Vis and/or fluorescence spectrometry
- Determination of the oxidation-reduction behavior of organic or inorganic catalysts (in solution) and materials (in solid state)
- Study of interactions at the electrode/electrolyte interface such as interactions of the solid with electrolytes of interest in solution (molecules of biological interest such as proteins, amino acids or DNA).
- Analysis by differential pulse voltammetry (DPV), cyclovoltammetry (CV) or linear scan voltammetry (LSV) among others, with the possibility of doing it under a nitrogen atmosphere or other gases of interest.
Available equipment
Equipment for sample preparation:
- JP Selecta 75L Presoclave Autoclave
- Nabertherm muffle (30-3000ºC)
- Parr Instrument PS131 Reactor
- Bandelin Ultrasound Probe SONOPULS HD 3100
- Laboratory freeze dryer Lyobench Model -55ºC NOXAIR
- IKA T20 Basic Ultraturrax
- Supelco Visiprep and Scharlau Extravac 12 vacuum SPE equipment
- DigiVOL syringe model eZY-Connect E-PREP for automatic microextraction
Equipment for catalytic studies
- Multireactor with 4-position stirring for simultaneous catalytic tests (Heidolph RZR-1)
- 3L Glass Catalytic Reactor with Temperature Control for Scale-Up of Catalytic and Photocatalytic Processes
- UV (36 W) and LED (5 W) photocatalytic reactors for multiple simultaneous reactions.
- Xenon light source, 300 W ozone-free with UV and visible filter for high-power photocatalytic reactions.
Instrumental Technique for analysis and characterization:
- Micromeritis ASAP 2020 gas physisorption equipment
- Varian ProStar 1200 Triple Quadrupole Mass Spectrometry Detector-Coupled Liquid Chromatograph (HPLC-TQ-MS/MS) (varies)
- ultra-high performance liquid chromatograph (Dionex UltiMate 3000, Thermo Scientific) coupled to ion trap mass spectrometry detector (UHPLC-IT-MS/MS) amaZon SL, Bruker
- EVOQ ELITE Bruker ultra-high performance liquid chromatograph coupled to triple quadrupole mass spectrometry detector (UHPLC-TQ-MS/MS)
- Agilent 1260 Infinity II HPLC High Performance Liquid Chromatograph with Fluorescence Diode Array Detector (HPLC-DAD-FLD)
- Cary 60 UV-Vis Spectrometer, Agilent Technologies
- Autolab Potentiostat-Galvanostat PGSTAT 128N, Metrohm
- Model Bipotentiostats μSTAT200 with connector sensor model DRP-DSC Dropsens (2 units) and flow cell DRP-FLWCL 70116
- Jasco UV-vis spectrophotometer
- FTIR Perkin-Elmer Spectrum Two-DTGS
- High Resolution LC Perkin-Elmer 300 with DAD detector
- Perkin-Elmer FL8500 Spectrofluorimeter with Integrating Ball for Liquid and Solid State Measurements
- Autolab Potentiostat-Galvanostat PGSTAT302 Metrohm
- STAT-I-400S Potentiostat/Galvanostat/Impedance μStat-i 400S.
- GC Perkin-Elmer Clarus 580 with dual channel with FID detector and MS (electronic impact) detector.
laboratory staff
Responsible of the lab. Mª Isabel Sierra Alonso ()
Technical support: Sonia Morante Zarcero ()
Areas: Sample preparation / Separation techniques (HPLC-DAD; HPLC-FL; (U)HPLC-MS/MS) / Electrochemical Techniques
Technical support: Damián Pérez Quintanilla ()
Areas: Characterization techniques (textural properties and UV-Vis tests)
Technical support: Diana Díaz García ()
Areas: Sample preparation / Separation techniques (GC-FID, GC-MS) / Characterization techniques (IR, UV and fluorescence spectroscopy)
Technical support: Josefa Ortiz Bustos ()
Areas: Sample preparation / Electrochemical techniques